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er membrane targeting signal fused miniturbo promiscuous biotin ligase er mtb  (Addgene inc)


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    Structured Review

    Addgene inc er membrane targeting signal fused miniturbo promiscuous biotin ligase er mtb
    Er Membrane Targeting Signal Fused Miniturbo Promiscuous Biotin Ligase Er Mtb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+membrane/pCDNA3-mouse+PKA-RIIalpha-mEGFP+(Plasmid+%2345527)/pmc10990152__pnas__2314947121__sapp-4-7-20
    Average 92 stars, based on 8 article reviews
    er membrane targeting signal fused miniturbo promiscuous biotin ligase er mtb - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Nevan Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-Spike-FLAG (VG40069-CF), pCMV3-FLAG-HKU1-Spike (VG40021-NF), pCMV3-NL63-Spike-FLAG (VG40604-CF), and pCMV3-OC43-Spike-FLAG (VG40607-CF) were purchased from SinoBiologicals. .. The pcDNA3-DYKDDDDK-SARS-CoV-2-PLpro plasmid contains a codon-optimized ORF for the PLpro domain (nucleotides 4955–5902, GenBank NC_045512) that was synthesized by GenScript then cloned between the Kpn I and Bam HI sites of pcDNA3.1(+).

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), and two-proline stabilizing mutants, K986P and V987P (S-2P) ( – ) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Neven Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-FLAG was purchased from SinoBiologicals (VG40069-CF). pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* ( ). pLJM1-ACE2-BSD was cloned by from cDNA of generated from Caco-2 cells. ..

    Generated:

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Nevan Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-Spike-FLAG (VG40069-CF), pCMV3-FLAG-HKU1-Spike (VG40021-NF), pCMV3-NL63-Spike-FLAG (VG40604-CF), and pCMV3-OC43-Spike-FLAG (VG40607-CF) were purchased from SinoBiologicals. .. The pcDNA3-DYKDDDDK-SARS-CoV-2-PLpro plasmid contains a codon-optimized ORF for the PLpro domain (nucleotides 4955–5902, GenBank NC_045512) that was synthesized by GenScript then cloned between the Kpn I and Bam HI sites of pcDNA3.1(+).

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), and two-proline stabilizing mutants, K986P and V987P (S-2P) ( – ) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Neven Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-FLAG was purchased from SinoBiologicals (VG40069-CF). pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* ( ). pLJM1-ACE2-BSD was cloned by from cDNA of generated from Caco-2 cells. ..

    Polymerase Chain Reaction:

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Nevan Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-Spike-FLAG (VG40069-CF), pCMV3-FLAG-HKU1-Spike (VG40021-NF), pCMV3-NL63-Spike-FLAG (VG40604-CF), and pCMV3-OC43-Spike-FLAG (VG40607-CF) were purchased from SinoBiologicals. .. The pcDNA3-DYKDDDDK-SARS-CoV-2-PLpro plasmid contains a codon-optimized ORF for the PLpro domain (nucleotides 4955–5902, GenBank NC_045512) that was synthesized by GenScript then cloned between the Kpn I and Bam HI sites of pcDNA3.1(+).

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), and two-proline stabilizing mutants, K986P and V987P (S-2P) ( – ) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Neven Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-FLAG was purchased from SinoBiologicals (VG40069-CF). pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* ( ). pLJM1-ACE2-BSD was cloned by from cDNA of generated from Caco-2 cells. ..

    Clone Assay:

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Nevan Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-Spike-FLAG (VG40069-CF), pCMV3-FLAG-HKU1-Spike (VG40021-NF), pCMV3-NL63-Spike-FLAG (VG40604-CF), and pCMV3-OC43-Spike-FLAG (VG40607-CF) were purchased from SinoBiologicals. .. The pcDNA3-DYKDDDDK-SARS-CoV-2-PLpro plasmid contains a codon-optimized ORF for the PLpro domain (nucleotides 4955–5902, GenBank NC_045512) that was synthesized by GenScript then cloned between the Kpn I and Bam HI sites of pcDNA3.1(+).

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), and two-proline stabilizing mutants, K986P and V987P (S-2P) ( – ) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Neven Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-FLAG was purchased from SinoBiologicals (VG40069-CF). pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* ( ). pLJM1-ACE2-BSD was cloned by from cDNA of generated from Caco-2 cells. ..

    Cloning:

    Article Title: Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions
    Article Snippet: .. A Spike mutant lacking the Furin cleavage site between S1 and S2 (P681S, R682G, R683G, R685del), and two-proline stabilizing mutants, K986P and V987P (S-2P) ( – ) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M) and pcDNA3-Envelope (E) were cloned from pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro and pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro (kind gifts from Neven Krogan, available from Addgene #141386 and #141385) using PCR to remove the 2xStrep epitope tag. pCMV3-MERS-FLAG was purchased from SinoBiologicals (VG40069-CF). pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* ( ). pLJM1-ACE2-BSD was cloned by from cDNA of generated from Caco-2 cells. ..



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